Application

Immunohistochemistry: 1:5-1:125

Western blot: 1:50-1:100

Optimal working dilutions must be determined by end user.

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.

Immunogen

Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).

Epitope: POU-domain protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.

Target description

46 kDa